The overall aim of the UKSOLAS project DOGEE-SOLAS is the parameterization of air-sea gas exchange, which is a currently a major uncertainty in global modelling. RRS Discovery cruise D320 thus was primarily concerned with the measurement of air-sea gas transfer velocities and some of the important physical parameters that influence them through contributing to near surface turbulence, and other processes. In brief, specific cruise objectives were: 1. Determine open ocean gas transfer velocities through a number of dual-tracer releases (3He & SF6). 2. Investigate the role of surfactant in gas exchange through a targeted surfactant release in conjunction with (1). 3. Determine CO2 fluxes and transfer velocities, and make associated hydrographic and turbulence related measurements from autonomous ASIS (Air-Sea Interaction Spar) buoys. 4. Measure air-sea fluxes of CO2, sensible heat, latent heat and momentum using the AUTOFLUX automated sensor array 5. Measure underway, total gas tension, dissolved O2, and CO2 to obtain independent air-sea gas exchange estimates. 6. Make covariance and gas budgeting estimates of air-sea gas exchange using intelligent profiling Lagrangian floats. 7. Independently determine DMS fluxes and gas transfer velocities for comparison with CO2 to examine the role of gas solubility in gas transfer. 8. Deploy a spar buoy (NOC) for measurement of wave heights and bubble properties 9. Record and measure whitecap coverage and wave breaking coincident with the air-sea flux measurements using ship mounted cameras 10. Record key meteorological variables 11. Quantify flow distortion biases in the direct flux measurements via the use of established models. 12. Make biogas, surfactant and bacterial measurements in the surface microlayer and in the uppermost metres of the ocean using surface microlayer samplers, a remotely operated catamaran, and a near surface sampler. 13. Deploy an autonomous powered profiler (ASIP) for turbulence-related measurements in the mixed layer. Objectives 1-12 were all met. Unfortunately, due to mechanical failure and loss of ASIP, objective 13 was not realised.

The cruise D361 forms part of a study entitled 'Physical and chemical forcing of diazotrophy in the (sub)-tropical Atlantic Ocean'. The study is investigating the potential influence of iron and phosphorus availability on nitrogen fixation in regions of the tropical Atlantic Ocean. The cruise also undertakes deep trace metal clean CTD casts as part of the International GEOTRACES programme. The purpose of the cruise therefore is to undertake measurements of dissolved and particulate iron and phosporpus availability, their spatial and temporal variations, and their impact on diazotrophy in the surface ocean. Aim: To quantify the supply and determine the biogeochemical cycling of Fe and other nutrients, and relate this to N2 fixation, diazotroph species distribution and N* fields. 1. Quantify the distribution of nutrients and trace metals: Quantify surface water and water column distributions of dissolved inorganic/organic N, P, Fe, and DAl, DMn and particulate P, N, Fe, Al, Mn. 2. Quantify the rate of Fe, Al, Mn, P and N supply to surface waters: Assess the source fluxes of the key elements for diazotrophs and source tracers to the surface ocean from atmospheric deposition and internal transport via diapycnal mixing and lateral advection. 3. Identify the source of subsurface Fe enrichment: Identify whether Fe-rich subsurface waters of the tropical North Atlantic thermocline originate from the atmosphere or the shelf using Fe distributions and Al, Mn, and O2 source tracers. 4. Quantify the diazotrophic response to Fe, phosphate, DOP supply: Relate the spatial distributions of inorganic Fe and organically complexed Fe, and phosphate and DOP to diazotrophy. The specific uptake of Fe, phosphate and DOP by the whole microbial community and Trichodesmium will be assessed by shipboard incubations, radiotracer techniques and enzyme bioassays. In addition, we will identify the connection between N2 fixation rates and diazotroph community structure, by comparing size fractionated 15N2-derived rates of N2 fixation (Fig. 1) with abundance and diversity of diazotrophs using nifH phylogeny. Objective 5: Investigate how the large scale transport pathways of Fe and P influence the N* distribution: Use fine-scale isopycnic model to reveal the large-scale transport pathways of Fe and P in the (S)-T Atlantic, and their effect on the N* distribution.