There were five main objectives for the trials cruise: The first tests of the Autosub Long Range AUV, testing of the HyBIS video guided grab system, testing of the MYRTLE-X Lander systems, testing of a deep camera system for the Lake Ellsworth probe and test deployments of the PELAGRA neutrally buoyant sediment capture drifters. The working area was about 300 miles south west of the Canary Islands, in international waters, over benthic plains of 4000 m depth, with some tests of the video systems over a isolated sea mount rising to 1200 m depth. Most of the objectives of the cruise where met, with successful diving and control of the Autosub LR, tests of the HyBIS and Ellsworth camera systems, and 3 test deployments and recoveries of two PELAGRA floats. Several wire tests of MYRTLE-X systems were carried out, predominantly successful, but concerns over the release system prevented a deployment of the lander. The tests were all purely engineering, hence no science data were collected.

CTD stations on 20W line from 50N to 20N, following the old WOCE line A16N Physical measurements (CTDO and LADCP) and discrete samples for salt, oxygen, inorganic nutrients and alkalinity/inorganic carbon Underway ADCP, surface ocean measurements, surface meteorology measurements. Contribution to GO-SHIP sustained hydrography program. Partial repeat of a hydrographic line last occupied in 2003.

The cruise D361 forms part of a study entitled 'Physical and chemical forcing of diazotrophy in the (sub)-tropical Atlantic Ocean'. The study is investigating the potential influence of iron and phosphorus availability on nitrogen fixation in regions of the tropical Atlantic Ocean. The cruise also undertakes deep trace metal clean CTD casts as part of the International GEOTRACES programme. The purpose of the cruise therefore is to undertake measurements of dissolved and particulate iron and phosporpus availability, their spatial and temporal variations, and their impact on diazotrophy in the surface ocean. Aim: To quantify the supply and determine the biogeochemical cycling of Fe and other nutrients, and relate this to N2 fixation, diazotroph species distribution and N* fields. 1. Quantify the distribution of nutrients and trace metals: Quantify surface water and water column distributions of dissolved inorganic/organic N, P, Fe, and DAl, DMn and particulate P, N, Fe, Al, Mn. 2. Quantify the rate of Fe, Al, Mn, P and N supply to surface waters: Assess the source fluxes of the key elements for diazotrophs and source tracers to the surface ocean from atmospheric deposition and internal transport via diapycnal mixing and lateral advection. 3. Identify the source of subsurface Fe enrichment: Identify whether Fe-rich subsurface waters of the tropical North Atlantic thermocline originate from the atmosphere or the shelf using Fe distributions and Al, Mn, and O2 source tracers. 4. Quantify the diazotrophic response to Fe, phosphate, DOP supply: Relate the spatial distributions of inorganic Fe and organically complexed Fe, and phosphate and DOP to diazotrophy. The specific uptake of Fe, phosphate and DOP by the whole microbial community and Trichodesmium will be assessed by shipboard incubations, radiotracer techniques and enzyme bioassays. In addition, we will identify the connection between N2 fixation rates and diazotroph community structure, by comparing size fractionated 15N2-derived rates of N2 fixation (Fig. 1) with abundance and diversity of diazotrophs using nifH phylogeny. Objective 5: Investigate how the large scale transport pathways of Fe and P influence the N* distribution: Use fine-scale isopycnic model to reveal the large-scale transport pathways of Fe and P in the (S)-T Atlantic, and their effect on the N* distribution.